Method and apparatus for electrophoresis separation and detection
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United States of America Patent
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Oct 17, 2000
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N/A
app pub date -
Jun 23, 1997
filing date -
Jun 28, 1996
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Abstract
An electrophoresis separation and detection apparatus for separating and detecting fluorophore-labeled samples by electrophoresis, which comprises a migrating and separating means for electrophoresis separation of the samples labeled with two or more kinds of fluorophore labels, respectively; an irradiating means which casts two or more kinds of exciting lights having different wavelengths on a plurality of positions in all the electrophoresis lanes or on the extensions of all the electrophoresis lanes substantially at the same time; and a detecting means which detects the samples separated by the electrophoresis, at the positions on which the exciting lights are casted, wherein each fluorophore label is composed of a substance for excitation which is excited by the exciting light and a substance for emission which emits light owing to energy transfer from the substance for excitation; two or more kinds of substances are used as each of the substance for excitation and the substance for emission; and the detecting means detects the samples by distinguishing the two or more kinds of the fluorophore labels from one another on the basis of the difference of combination of the two or more kinds of the substances used as the substance for excitation and those used as the substance for emission.
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