Method for measuring the concentration or the activity of protease inhibitor
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United States of America Patent
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Oct 10, 2000
Grant Date -
N/A
app pub date -
Aug 18, 1998
filing date -
Aug 29, 1997
priority date (Note) -
Expired
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Abstract
A method for measuring the concentration or the activity of UTI quickly and easily at high sensitivity. A urine sample, a buffer solution, a trypsin solution and a substrate solution are mixed and the trypsin activity is then measured. Thus, the UTI concentration in the urine sample is determined. In this case, a substrate solution having only L-BAPNA is used as the substrate, and the surfactant is mixed in at least one selected from the buffer solution and the enzyme solution. The mixing ratio of the surfactant is about 1 wt. % in the entire enzyme reaction solution. Examples of the surfactant include polyoxyethylene (40) octylphenylether, polyoxyethylene (10) octylphenylether, 3-[(3-cholamido propyl)dimethylammonio]-propanesulfonic acid, 3-[(3-cholamido propyl)dimethylammonio]-2-hydroxypropanesulfonic acid, and polyoxyethylene sorbitan monolaurate. As shown in FIG. 1, the sensitivity improves when using L-BAPNA.
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