Potent antigen presenting cell composition
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United States of America Patent
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Sep 19, 2000
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N/A
app pub date -
Jul 12, 1995
filing date -
Jul 12, 1995
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Abstract
A method of obtaining a blood-cell fraction enriched for potent antigen presenting cells is disclosed. The method includes obtaining a monocyte-depleted lymphocyte fraction, culturing the cell fraction in a serum-free medium for a period sufficient to produce a morphological change in dendritic-precursor cells to cells having the morphology of dendritic cells, harvesting non-adherent cells produced by said culturing, and enriching the portion of dendritic cells in the harvested cells by density centrifugation. Also disclosed is a PAP cell composition containing cells enriched for PAP activity in a collagen matrix.
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