Nucleic acid probe molecule of hairpin-shape structure and method for detecting nucleic acids using the same
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United States of America Patent
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Sep 5, 2000
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N/A
app pub date -
Nov 13, 1997
filing date -
Nov 14, 1996
priority date (Note) -
Expired
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Abstract
This invention provides a hairpin-shaped nucleic acid probe which is capable of forming stable hybrid with target nucleic acid, and a method for detecting nucleic acid containing a sequence complementary to a part of sequence of the probe. After contacting RecA to a hairpin-shaped single strand probe for hybridization, this complex is contacted with a target nucleic acid to form a triplex recombination intermediate using RecA function. After ligating the probe and the target nucleic acid, the target nucleic acid is detected by the label on the probe.
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