Rapid detection method of Mycobacterium bovis by fluorescence polarization
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United States of America Patent
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Aug 29, 2000
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app pub date -
Jun 27, 1997
filing date -
Jun 28, 1996
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Abstract
An assay for the detection of antibodies to the MPB70 protein secreted by Mycobacterium bovis utilizes a tracer consisting of the MPB70 protein conjugated to a fluorophore such as fluorescein. Upon mixing with the antibodies specific for MPB70 protein contained in the serum of an animal infected with M. bovis, the bound tracer exhibits an increase in fluorescence polarization, detectable in an instrument.
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