Preparation of lipid-nucleic acid particles using a solvent extraction and direct hydration method
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United States of America Patent
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Aug 29, 2000
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Jul 23, 1998
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Jul 24, 1997
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Abstract
This invention relates to a novel Solvent Extraction and Direct Hydration (SEDH) method for preparing lipid-nucleic acid particles which are useful for the introduction of nucleic acids (e.g., plasmid DNA, antisense molecules, ribozymes, etc.) into cells. The lipid-nucleic acid particles prepared using the methods of the present invention have enhanced circulation characteristics and serum stability and, thus, they are extremely effective as nucleic acid delivery vehicles.
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