Cell-free system for initiation of DNA replication
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United States of America Patent
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Aug 22, 2000
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N/A
app pub date -
Jan 25, 1999
filing date -
Jun 26, 1996
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Expired
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Abstract
A cell-free system for initiating DNA replication under cell cycle control includes S phase cytosol or a fraction thereof in which are incubated G1 phase nuclei, which are co-incubated with S phase nuclei or a fraction thereof and/or cyclins A and/or E complexed to their cognate cyclin dependent kinase (Cdk2). The system may be used to assay for substances which modulate DNA synthesis or initiation thereof, and which have therapeutic potential in a number of contexts.
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