Procedure for the genetic transformation of adult plants of woody species
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United States of America Patent
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Aug 15, 2000
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app pub date -
Mar 5, 1998
filing date -
Mar 5, 1997
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Abstract
The procedure is based on the use, as vegetable starting material for the transformation, of the first shoots from the graft of buds of adult trees onto stock, the genetic transformation of explants from the adult shoots by mean of co cultivation with Agrobacterium tumefaciens in mother plaques, and the obtaining of complete adult woody plants by means of in vitro micrografting of the transgenic buds, apices or shoots, regenerated by means of the explants, by means of in vitro micrograft onto stock cultivated in vitro. This procedure makes it possible to avoid the juvenile period and the high heterozygosis which affects most woody species, blossoming and fruiting of the transgenic plants are brought forward, and it permits the direct genetic transformation of commercially interesting varieties. It has argicultural applications.
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