Spodoptera frugiperda single cell suspension cell line in serum-free media, methods of producing and using

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United States of America Patent

PATENT NO 6103526
SERIAL NO

09169178

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Abstract

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Disclosed and claimed is a new insect cell line, Sf900+, ATCC CRL-12579. The insect cell line was established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 (ATCC CRL-1771) through multiple rounds of limiting dilution and selection in a serum-free insect medium supplemented with added human insulin. The insect cell line is useful in BEVS or as an adjuvant and has many characteristics and advantages. Also disclosed and claimed are recombinant proteins from recombinant baculovirus expression in insect cells such as Sf900+ cells, for instance, HA, NA, EPO, CD4, CEA, and thrombospondin.

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Patent Owner(s)

Patent OwnerAddress
PROTEIN SCIENCES CORPORATION1000 RESEARCH PARKWAY MERIDEN CT 06450-7159

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
DeBartolomeis, James Millbury, MA 2 53
Foellmer, Harald G Guilford, CT 1 32
Knell, John Meriden, CT 4 98
Smith, Gale E Wallingford, CT 10 683
Voznesensky, Andrei I West Hartford, CT 10 314

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