Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
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United States of America Patent
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Aug 15, 2000
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N/A
app pub date -
Oct 7, 1997
filing date -
Oct 7, 1997
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Expired
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Abstract
A method for the rapid, high-sensitivity detection of bacteria in a malt beverage comprising liquid and solid components, wherein the method comprises the steps of: aseptically sampling said malt beverage; separating the solids in the sample from the liquid by means of a substrate having antibodies attached thereto; extracting DNA from the separated solids; subjecting the extracted DNA to a nested polymerase chain reaction, said reaction comprising: enzymatically amplifying at least one fragment of said extracted DNA using at least one highly conserved primer to produce a first amplified sample; enzymatically amplifying a product sequence from said first amplified sample, using a less highly conserved primer to produce a second amplified sample; examining said second amplified sample for the presence of DNA fragments associated with said bacteria.
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