Antitumor active substances
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United States of America Patent
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Jul 25, 2000
Grant Date -
N/A
app pub date -
Jun 14, 1999
filing date -
Dec 20, 1996
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Expired
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Abstract
By extracting hot water-insoluble and ethanol-insoluble components of the fruit body of Agaricus blazei Murill belonging to the genus Agaricus with 5% ammonium oxalate aqueous solution, degrading the extract with hydrochloric acid and then purifying the acid-degraded product through gel permeation and affinity chromatography, there are obtained the antitumor-active substances having a weight-average molecular weight of 38.times.10.sup.4 daltons and a dispersion degree of 2.3, a weight-average molecular weight of 29.times.10.sup.4 daltons and a dispersion degree of 7.3, a weight-average molecular weight of 2.4.times.10.sup.4 daltons and a dispersion degree of 4.1, and a weight-average molecular weight of 2.0.times.10.sup.4 daltons and a dispersion degree of 3.6. These substances exhibit a significant antitumor effect against solid tumor, when determined by gel permeation.
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