Automated on-line evaporation light scattering detection to quantify isolated fluid sample compounds in microtiter plate format
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United States of America Patent
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Jun 20, 2000
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N/A
app pub date -
Dec 22, 1998
filing date -
Dec 22, 1998
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Expired
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Abstract
Single pass methods and systems for measuring the total masses of individual compounds present in fluid samples in real time, and particularly for fluid samples prepared in microtiter plate format are provided. Individual compounds are isolated by fluid separation column (14). Portions of the individual compounds isolated by column (14) are analyzed by mass spectrometer (16), which determines their concentration on the basis of their molecular weights, and an evaporative light scattering detector (24), (which determines the total masses of each of the isolated compounds passing therethrough). Total collected masses of the individual compounds are calculated by determining the amount and portion of the isolated compounds diverted into evaporative light scattering detector (24) during a period of fraction collection.
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