DNA extraction buffer and method of use thereof
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United States of America Patent
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Jun 6, 2000
Issued Date -
N/A
app pub date -
Nov 12, 1997
filing date -
Nov 1, 1996
priority date (Note) -
Expired
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Abstract
Internal Transcribed Spacer (ITS) DNA sequences from the ribosomal RNA gene region are described for different species and strains of Helminthosporium carbonum, Helminthosporium turcicum, Helminthosporium maydis, Cercospora zeae-maydis, Kabatiella zeae and Puccinia sorghi. Specific primers from within these sequences are identified as being useful for the identification of the fungal isolates using PCR-based techniques. Also described is a novel extraction buffer solution for use in isolating DNA from an organism.
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