Global amplification of nucleic acids
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United States of America Patent
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May 23, 2000
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N/A
app pub date -
Apr 29, 1998
filing date -
Aug 29, 1996
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Expired
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Abstract
Nucleic acid starting material composed of a collection of single stranded nucleic acid molecules is anchored and processed by a direction random printing method to produce a mixture of shorter random size DNA molecules well-suited for achieving substantially uniform global PCR amplification. The processing and global amplification method disclosed is especially useful in conjunction with subtractive hybridization procedures applied, for example, to the study of differential gene expression.
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