Methods for the lyophilization of platelets, platelet membranes or erythrocytes
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United States of America Patent
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May 9, 2000
Issued Date -
N/A
app pub date -
Apr 15, 1998
filing date -
Jun 14, 1996
priority date (Note) -
Expired
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Abstract
The present invention provides methods for preserving living biological materials by lyophilization that enable cells and tissues to be stored for extended periods of time with minimal loss of biological activity. In one embodiment, the inventive methods comprise contacting a biological material with a preservative solution comprising trimethyl amine oxide, sodium citrate and sodium chloride, reducing the temperature of the biological material to less than 0.degree. C., and drying the biological material to provide a freeze-dried material. The preservative solutions employed in the inventive methods are preferably isotonic with the material to be preserved and substantially free of iodide, dihydrogen phosphate, bicarbonate, nitrate and bisulfate.
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