Bilayer membrane device
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United States of America Patent
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May 2, 2000
Grant Date -
N/A
app pub date -
May 29, 1997
filing date -
May 30, 1996
priority date (Note) -
Expired
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Abstract
An alkanethiol monolayer membrane is formed on a substrate having an Au layer using Au--S bonding, and a second layer constructed by a monolayer lipid membrane is formed on its surface to form a BLM. A gel layer is formed using agarose on the side of the BLM having the second monolayer lipid layer. In addition, a polymer layer is formed using the amino acid poly-L-lysine between the surface of the monolayer lipid membrane and the gel layer to immobilize the BLM. The use of this supporting constitution for holding and immobilizing the membrane extends the life-span of the BLM, while the gel layer keeps the electrolyte solution required for maintaining flexibility of the membrane and activating protein on the surface of the BLM. In addition, a BLM having a life-span of one month or more is obtained by arranging a membrane supporting portion that minimizes lifting of the membrane molecules due to buoyancy above the BLM arranged upright in an aqueous system.
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