Thermostable ligase mediated DNA amplification system for the detection of genetic diseases
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United States of America Patent
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Apr 25, 2000
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Oct 7, 1997
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May 3, 1990
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Abstract
The present invention relates to the cloning of the gene of a thermophilic DNA ligase, from Thermus aquaticus strain HB8, and the use of this ligase for the detection of specific sequences of nucleotides in a variety of nucleic acid samples, and more particularly in those samples containing a DNA sequence characterized by a difference in the nucleic acid sequence from a standard sequence including single nucleic acid base pair changes, deletions, insertions or translocations.
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