Production of C-terminal amidated peptides from recombinant protein constructs
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United States of America Patent
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Apr 18, 2000
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app pub date -
Sep 19, 1997
filing date -
Dec 7, 1994
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Abstract
A method for the production of C-terminal amidated recombinant peptides is provided. The method employs a recombinant protein construct having multiple copies of a target peptide linked by intraconnecting peptides. The intraconnecting peptides permit the multicopy construct to be selectively reacted to produce product peptides having a C-terminal .alpha.-carboxamide. A recombinant gene containing a DNA sequence coding for the recombinant protein construct and an expression cassette, an expression vector and a transformed cell including the recombinant gene are also provided.
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