Method for preparing an influenza virus, antigens obtained and applications thereof
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United States of America Patent
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Apr 11, 2000
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N/A
app pub date -
May 27, 1997
filing date -
Aug 16, 1994
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Abstract
Method for preparing purified influenza antigens from a fluid containing influenza virus, comprising the steps of concentration, purification, fragmentation and, where appropriate, inactivation, characterized in that: A. either the purification step entails several ultracentrifugation steps separated by a filtration step, B. or the fragmentation step is performed on the live virus in the presence of an amphiphilic nonionic detergent, followed by a removal of undesirable substances by filtration, retaining all of the viral constituents, or these two steps are carried out in any order.
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