Method for measuring the concentration of polynucleotides
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United States of America Patent
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Mar 28, 2000
Grant Date -
N/A
app pub date -
Oct 21, 1997
filing date -
Oct 25, 1996
priority date (Note) -
Expired
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Abstract
A method for measuring the concentration of polynucleotide in a sample comprising the steps of: (1) preparing a sample, a Raman scattering active agent capable of bonding to a polynucleotide and a surface-enhanced Raman scattering (SERS) active substrate capable of capturing the agent; (2) mixing the sample, the agent and the substrate to capture the agent which has not been bonded to polynucleotide on the surface of the substrate; (3) irradiating the mixture; and (4) measuring the SERS radiation which is generated by the agent captured on the surface of the substrate.
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