Non-competitive immunoassay with blocking of unoccupied specific binding sites on solid phase
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United States of America Patent
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Mar 14, 2000
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N/A
app pub date -
Jul 8, 1998
filing date -
Feb 6, 1996
priority date (Note) -
Expired
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Abstract
A non-competitive method for the determination of analytes. Initially the analyte is bound to a specific binding partner, after which the unoccupied binding sites of the binding partner are inactivated. The bound analyte is then dissociated from the binding partner and replaced by a labeled marker, after which the bound labeled marker is determined. The signal from the bound labeled marker is directly proportional to the initial amount of analyte in the sample, which makes the present method more favorable than the competitive assays.
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