Wavelength-shifting probes and primers and their use in assays and kits
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United States of America Patent
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Mar 14, 2000
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app pub date -
Jul 28, 1998
filing date -
Jul 28, 1998
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Abstract
Hairpin-forming oligonucleotide probes and primers triple-labeled with a pair of florophores, a shorter wavelength harvester and a longer wavelength emitter, and a quencher. When the probes and primers are stimulated by light that excites the harvester, opening causes an increase in fluorescence by the emitter, while fluorescence from the harvester is continually suppressed. The probes and primers may be used for detection of nucleic acid targets in assays, including amplification assays. Assay kits are provided.
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