Method for the separation of recombinant pro-factor IX from recombinant factor IX
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United States of America Patent
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Mar 7, 2000
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N/A
app pub date -
Oct 4, 1995
filing date -
Oct 4, 1994
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Abstract
A method for separation of recombinant pro-Factor IX from recombinant Factor IX by a chromatographic method is described. According to a preferred embodiment, the mixture of both proteins is bound to an ion exchanger and pro-Factor IX and Factor IX are eluted separately from each other by buffer solutions with different salt concentrations and/or pH values. According to the method of the invention, pro-Factor IX and Factor IX are obtainable in highly pure form and free from the other factor respectively.
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