Method for suppressing nonspecific hybridization in primer extension method
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United States of America Patent
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Mar 7, 2000
Grant Date -
N/A
app pub date -
May 30, 1997
filing date -
Dec 9, 1994
priority date (Note) -
Expired
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Abstract
An object of the present invention is to suppress nonspecific extension reaction of the primer in the primer extension method. The primer extension reaction to form a nucleic acid strand complementary to a nucleic acid template strand with the use of a primer according to the present invention is characterized in that the reaction between the primer and the template strand is carried out in the presence of a nucleic acid or a derivative thereof which is complementary to said primer and has an affinity for said primer is equivalent to or less than that of said primer for the nucleic acid template strand.
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