Dry analytical element for acetaminophen assay
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United States of America Patent
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Jan 18, 2000
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N/A
app pub date -
Jul 15, 1992
filing date -
Jul 15, 1992
priority date (Note) -
Expired
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Abstract
A spectrophotometric assay for the detection of acetaminophen in aqueous fluids can be carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; a second enzyme, ascorbic acid oxidase, to oxidize the p-aminophenol so that it couples to a water soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.
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