Method for in vitro protein synthesis
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United States of America Patent
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Dec 7, 1999
Issued Date -
N/A
app pub date -
Jan 13, 1999
filing date -
Nov 8, 1994
priority date (Note) -
Expired
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Abstract
A method for performing coupled in vitro transcription and translation reactions is disclosed. In the transcription reaction, the quantity of mRNA produced is limited to a level of less than about 2.5 micrograms in a 10 microliter volume prior to the translation elements being added. Limiting the level of mRNA produced prevents saturation of the translational processes and thus aids in the efficiency and fidelity of the translation process.
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