In planta method for the production of transgenic plants
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United States of America Patent
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Nov 30, 1999
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Oct 20, 1997
filing date -
Oct 20, 1997
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Abstract
The present invention relates to a method for producing a transgenic plant in which a transforming agent such as a recombinant Agrobacterium or an isolated DNA molecule capable of conferring a desired phenotypic trait is injected into plant tissues using a needleless injection device. A preferred embodiment of the method provides the precise delivery of the transforming agent to floral tissues of a plant, allowing the direct insertion of the DNA of the transforming agent into germline cells of the plant that develop into seeds. This embodiment provides a more efficient method of transformation and subsequent regeneration of a transgenic plant.
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