Recovery of and uses of specific binding agents
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United States of America Patent
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Nov 23, 1999
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N/A
app pub date -
Oct 22, 1997
filing date -
Nov 24, 1994
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Abstract
An antibody specific for a target analyte is purified by affinity chromatography on a substrate bearing a low-affinity analogue of the target analyte. The antibody is displaced from the substrate by contact with a second analogue of intermediate affinity, which remains complexed with the antibody. This complex can be used in a conventional assay for the target analyte, which displaces the intermediate-affinity analogue. The complexed antibody is rendered more storage-stable because the second analogue protects the antibody binding reagion.
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