Methods for agrobacterium-mediated transformation

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United States of America Patent

PATENT NO 5981840
SERIAL NO

08788018

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Abstract

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The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

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Patent OwnerAddress
PIONEER HI-BRED INTERNATIONAL INC7100 NW 62ND AVENUE JOHNSTON IA 50131-1014

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cai, Tishu Urbandale, IA 6 378
Gu, Weining Urbandale, IA 17 364
Pierce, Dorothy A Urbandale, IA 10 305
Zhao, Zuo-Yu Urbandale, IA 36 472

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  • 247 Citation Count
  • C12N Class
  • 98.74 % this patent is cited more than
  • 26 Age
Citation count rangeNumber of patents cited in rangeNumber of patents cited in various citation count ranges171787425836111401 - 1011 - 2021 - 3031 - 4041 - 5051 - 6071 - 8081 - 9091 - 100100 +0102030405060708090100110120130140150160170180190

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