Methods for agrobacterium-mediated transformation

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United States of America Patent

PATENT NO 5981840
SERIAL NO

08788018

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Abstract

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The present invention relates to methods for improving the transformation frequency of Agrobacterium-mediated transformation of maize embryos. A preferred method for transforming maize using Agrobacterium comprises the steps of: contacting at least one immature embryo from a maize plant with Agrobacterium capable of transferring at least one gene to said embryo; co-cultivating the embryos with Agrobacterium; culturing the embryos in medium comprising N6 salts, an antibiotic capable of inhibiting the growth of Agrobacterium, and a selective agent to select for embryos expressing the gene; and regenerating plants expressing the gene.

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Patent Owner(s)

Patent OwnerAddress
PIONEER HI-BRED INTERNATIONAL INC7100 NW 62ND AVENUE PO BOX 1014 JOHNSTON IA 50131-1014

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cai, Tishu Urbandale, IA 6 378
Gu, Weining Urbandale, IA 17 364
Pierce, Dorothy A Urbandale, IA 10 305
Zhao, Zuo-Yu Urbandale, IA 36 472

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