Process for the purification of proteins
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United States of America Patent
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Nov 9, 1999
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N/A
app pub date -
Jul 22, 1997
filing date -
Jun 7, 1995
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Abstract
The present invention is directed to a simple and efficient process for the recovery of a biologically active glycoprotein from a biological fluid containing it. In a preferred embodiment, it comprises: a) contacting a filtered culture fluid with a dihydroxyboronyl bearing chromatographic support b) eluting with a first eluting buffer and contacting this eluate directly with an anion exchange matrix bearing quaternary ammonium functional groups c) eluting with a second eluting buffer and collecting the product therefrom.
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