Production of proteins using homologous recombination
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United States of America Patent
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Nov 9, 1999
Issued Date -
N/A
app pub date -
Jun 6, 1995
filing date -
Nov 6, 1989
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Abstract
Methods and compositions are provided for expression of mammalian genes in culture. An amplifiable gene is introduced by homologous recombination in juxtaposition to a target gene, the resulting combination of amplifiable gene and target gene transferred to a convenient host and the target gene amplified by means of the amplifiable gene. The resulting expression host may then be grown in culture with enhanced expression of the target gene.
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