Process and apparatus for the genetic transformation of cells
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United States of America Patent
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-
Nov 2, 1999
Grant Date -
N/A
app pub date -
Dec 18, 1990
filing date -
Dec 19, 1989
priority date (Note) -
Expired
status (Latency Note)
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Abstract
In a tubular pressure chamber a drop of a DNA solution with gold particles suspended therein is atomized at the opening of a cannula by a pressure impact. The fog droplets containing the gold particles and the DNA entrained by them, are pressured by the pressure impact through a restriction at the end of the pressure chamber and thereby accelerated and focused. They subsequently traverse in free flight an evacuated specimen chamber and impact in a narrowly limited target area with a predetermined pulse, cells fixed on a holder, thereby penetrating them.
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