Yeast strains and modified albumins
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United States of America Patent
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Oct 12, 1999
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N/A
app pub date -
Nov 11, 1996
filing date -
Mar 5, 1994
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Abstract
Albumin, for example human albumin, is expressed and secreted in yeast which has been mutated to lack the yeast aspartyl protease 3 (Yap3p) or its equivalent, thereby reducing the production of a 45 kD albumin fragment. A further reduction is achieved by additionally deleting the Kex2p function. Alternatively, a modified albumin is prepared which is not susceptible to Yap3p cleavage, for example human albumin which is R410A, K413Q and K414Q.
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