Determination of a genotype of an amplification product at multiple allelic sites
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United States of America
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Oct 5, 1999
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Feb 4, 1998
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Feb 4, 1998
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Abstract
A method is provided for genotyping a target sequence at at least two allelic sites by a 5' nuclease amplification reaction. In one embodiment, the method includes performing a nucleic acid amplification on a target sequence having at least two different allelic sites using a nucleic acid polymerase having 5'->3' nuclease activity and a primer capable of hybridizing to the target sequence in the presence of two or more sets of allelic oligonucleotide probes wherein:
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