Method for classifying and counting immature leukocytes
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United States of America Patent
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Sep 28, 1999
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app pub date -
Nov 17, 1997
filing date -
Nov 20, 1996
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Abstract
A hematological sample is treated with a hemolytic agent that maintains immature leukocytes in a viable state and damages other leukocytes. The damaged leukocytes are stained with a fluorochrome which can stain damaged cells. At least one kind of scattered light and at least one kind of fluorescence of the treated and stained blood cells are measured to classify and count leukocytes based on the intensities of the scattered light and the fluorescence. Highly precise measurement of immature leukocytes and simultaneous classification and counting of normal leukocytes are achieved.
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