Regioselective .alpha.-hydrolysis of amino acid diesters using pig liver esterase
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United States of America Patent
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Jul 27, 1999
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app pub date -
Jan 26, 1998
filing date -
Aug 26, 1996
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Abstract
The regioselective and chemoselective hydrolysis of an .alpha.-ester group of an amino acid diester using pig liver esterase enzyme (PLE) is disclosed. The amino acid diesters may be either N-protected or unprotected and the diester groups may be the same or different. In particular, the preparation of a number of .gamma.-ester glutamates and .beta.-ester aspartates are provided.
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