Device for measuring ATP
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United States of America Patent
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Jun 29, 1999
Issued Date -
N/A
app pub date -
Aug 1, 1997
filing date -
Feb 19, 1997
priority date (Note) -
Expired
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Abstract
A method of taking a sample of an inoculated culture medium and measuring the amount of ATP using a luciferase/luciferin reaction. Additional samples are taken at later time intervals to determine whether anything is growing in the medium. Luciferase/luciferin reagent is immobilized on an absorbent tip of a sampling device in order to stabilize the luciferase/luciferin reagent and to provide a convenient means to combine the luciferase/luciferin reagent with the sample. A counting tube containing an extraction reagent is employed to extract intracellular ATP from the sample. Chlorhexadine diacetate (CDA) is the preferred extraction reagent.
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