Separation of molecules from dilute solutions using composite chromatography media having high dynamic sorptive capacity at high flow rates
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United States of America Patent
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May 25, 1999
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N/A
app pub date -
Nov 13, 1995
filing date -
Nov 13, 1995
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Abstract
This invention relates generally to modified porous solid chromatographic media and processes for the preparation and use of same. In particular, chromatographic media of porous mineral oxide, polymeric, or polymer-coated mineral oxide supports are disclosed which are characterized by a reversible high sorptive capacity and high intraparticle mass transfer rates. In order to prevent non-specific adsorption of or interaction with biomolecules, these supports may be passivated by use of a passivation mixture comprising a main monomer, a passivating monomer, and a crosslinking agent, which mixture upon polymerization results in substantial elimination of the undesirable non-specific interaction with biomolecules.
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