Method and apparatus for assaying enzymatic reaction
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United States of America Patent
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May 18, 1999
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N/A
app pub date -
Jun 5, 1998
filing date -
Feb 28, 1996
priority date (Note) -
Expired
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Abstract
A method and apparatus for measuring enzyme activity by supplying a substrate solution and an enzyme solution into a reaction vessel combined with a total reflection absorption prism is disclosed. A measurement solution (40) in which the enzyme solution and the substrate solution are combined together is kept at a constant temperature within the reaction vessel (26) by a temperature control unit (23), and is stirred by a stirrer (21). Infrared light (36) emitted from an infrared light source (3) is made incident on the interface between a total reflection absorption prism (28) disposed in contact with the measurement solution (40) and the measurement solution (40) from the side of the total reflection absorption prism (28) and is totally reflected by the interface. The spectrum of transmitted infrared light (37) thus totally reflected and emitted is detected by an infrared light detector (5). According to thus detected spectrum, a change in the infrared absorption spectrum or absorbance is determined, whereby the enzyme reaction in the measurement solution (40) is measured.
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