Method of detecting organisms in a sample
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United States of America Patent
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May 11, 1999
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N/A
app pub date -
Dec 5, 1997
filing date -
Dec 5, 1997
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Abstract
The presence or absence of an organism in a sample is detected by isolating the organism from the sample by a suitable affinity matrix, releasing the organism from the affinity matrix, rupturing the cells of the organism to release total nucleic acid and hydrolyzing or digesting the total nucleic acid to form mononucleotides or individual free nucleic acid basic and inorganic phosphate to form an analyte solution, and assaying the analyte solution for the at least one presence of free nucleic acid base or inorganic phosphate to thereby determine whether the organism was present in the sample.
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