DNA molecules encoding PDGF molecules having protease resistant basic cleavage sites
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United States of America Patent
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Apr 20, 1999
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Sep 9, 1994
filing date -
Jul 23, 1990
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Abstract
PDGF B-chain polypeptides characterized by the substitution or deletion of a lysine or arginine residue at position 27, 28, 32, 79, 80 or 81 of native B-chain are disclosed. Also disclosed are PDGF-like proteins containing the B-chain polypeptides. The polypeptides and proteins may be produced through the use of cultured cells transfected or transformed to express DNA molecules that encode the polypeptides. The PDGF-like proteins are useful as components of cell culture media and for accelerating the healing of wounds.
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