DNA encoding lemA-independent GacA and its use in activating gene expression
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United States of America Patent
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Apr 6, 1999
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Jan 20, 1998
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Dec 6, 1996
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Abstract
The present invention exploits the discovery that certain single base changes in the gacA gene coding sequence, which result in certain single amino acid changes in the encoded GacA protein, render it LemA-independent. The present invention therefore provides DNA that encodes mutant GacA proteins that do not require phosphorylation by LemA in order to be active as transcriptional activators.
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