Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing
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United States of America Patent
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Apr 6, 1999
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app pub date -
Jan 30, 1995
filing date -
Sep 15, 1993
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Abstract
This invention is directed to cryopreservation of harvested mammalian tissues and living cultured tissue equivalents made by in vitro technology. The invention involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution, agitating the cryoprotectant solution and the immersed tissue to achieve effective penetration of the cryoprotectant solution into the tissue, and then freezing the tissue at a very slow freezing rate at 0.3.degree. C. or less/min. In the freezing step, extracellular ice formation is initiated by seeding. The cryopreserved tissue may be stored for indefinite periods of time prior to use. The cultured tissue equivalent is an in vitro model of the equivalent human tissue, such as skin or cornea, which, when retrieved from storage can be used for transplantation or implantation in vivo or for screening compounds in vitro.
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