Isolation of cells from organ tissue using sonication
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United States of America Patent
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Mar 9, 1999
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N/A
app pub date -
May 17, 1995
filing date -
May 17, 1995
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Abstract
A method for isolating specific viable cell types from surrounding organ tissue is provided. The method entails the use of sonication in conjunction with tissue dissociating agents to free the cells of interest. A specific application of the method is the isolation of the insulin producing tissue of the pancreas, the islets of Langerhans. The method results in a high yield of islets that maintain a high level of viability.
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