Importance

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Abstract

Peptides or peptides having an amino acid-like moiety in place of an amino acid moiety thereof immobilized on glass beads, especially controlled-pore glass beads, by a C-terminal .alpha.-carboxyl-.omega.-amino linker and .alpha.-silyl-.omega.-amino anchor, especially wherein the anchor has the structural formula --NH(CH.sub.2).sub.n Si(OR).sub.2 O-- wherein n is 3 and R is hydrogen or the glass bead and the linker has the structural formula --?NH(CH.sub.2).sub.r CO!.sub.s -- wherein r is 5 and s is an integer from 1 through 10 and marked by an N-terminal fluorescent marker, especially wherein the N-terminal fluorescent marker is 3-(7-hydroxy-4-coumarinyl)propionyl, and method of preparation thereof and method of use thereof in carrying out biochemical assays, especially wherein the immobilized peptide or peptide having an amino acid-like moiety in place of an amino acid moiety thereof is cleaved by a protease and the resulting fluorescence in the supernatant solution is measured to determine the extent of cleavage, are disclosed.

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First Claim