Immunassay of non-A, non-B hepatitis virus-related antigens, monoclonal antibodies for use therein, and hybridomas producing the antibodies
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United States of America Patent
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Feb 16, 1999
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N/A
app pub date -
Jul 11, 1995
filing date -
Jul 12, 1994
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Abstract
This invention concerns a monoclonal antibody having binding specificity for an antigenic determinant site on core structural protein from Non-A,Non-B hepatitis virus (NANBV); a hybridoma cell line capable of producing the monoclonal antibody; a process for the preparation of the monoclonal antibody; an immunoassay of NANBV-related antigens by use of the monoclonal antibody; and a test kit for use in the immunoassay. The preferred monoclonal antibody is 5E3, 5F11, 515S or 1080S. The monoclonal antibody can specifically recognize the NANBV core structural protein in sera from patients with Non-A,Non-B hepatitis thereby being served extensively as an antibody in various immunological reagents for definitive diagnosis of Non-A, Non-B hepatitis.
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