Assay for inhibitors of DP-1 and other DP proteins
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United States of America Patent
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Feb 16, 1999
Grant Date -
N/A
app pub date -
Feb 26, 1996
filing date -
Jul 1, 1994
priority date (Note) -
Expired
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Abstract
The protein DP-1, part of the DP-1/E2F-1 transcription factor complex, as well as DP-2 and DP-3 has its phosphorylation level regulated during cell cycle progression. This finding allows assays to be based on changes in phosphorylation of DP proteins, in particular for agents which may affect the phosphorylation state of DP. DP-1 has been found to have a greater affinity to DNA when in a hypophosphorylated state. Antibodies that recognize phosphorylation sites on DP-1 are also disclosed.
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