Process for inactivating carboxypeptidase Y in hirudin-containing culture broths
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United States of America Patent
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Feb 2, 1999
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app pub date -
Aug 12, 1996
filing date -
Aug 16, 1995
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Abstract
The present invention relates to a process for inactivating Carboxypeptidase Y (CPY) in a hirudin-containing culture broth produced by fermenting a transformed yeast. It has been found that inactive precursors of CPY are activated at a temperature 70.degree. C. and that the active peptidases will further degrade hirudin when the two proteins are present together in a composition. The present invention overcomes the CPY activation problem by heating a broth containing hirudin and CPY to a temperature of about 80.degree.-100.degree. C. in about one minute or less.
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