Direct cloning of DNA fragments
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United States of America Patent
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Jan 5, 1999
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app pub date -
Jun 18, 1997
filing date -
Jun 18, 1997
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Abstract
A vector for the direct cloning of the products of PCR protocol incorporates single nucleotide overhangs at one or both ends of a linearized DNA segment. The single nucleotide overhangs are uracil or inosine residues, as desired, to facilitate cloning of the desired PCR products.
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