Primer extension mass spectroscopy nucleic acid sequencing method
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United States of America Patent
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Dec 15, 1998
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N/A
app pub date -
May 10, 1996
filing date -
Nov 17, 1993
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Abstract
A method of sequencing a target nucleic acid using primer extension mass spectroscopy to generate an observed mass spectrum, on which base calling cycles are carried out using calibrated inter-peak mass difference values such that: each called base allows mass calculation for the base-called peak in the observed mass spectrum; this calculated mass is then used as a further calibration point for subsequent rounds of base calling. A reaction mixture of all four base-specific chain extension nucleotides and four chain termination nucleotide analogues. A method of performing mass spectroscopy, which method comprises subjecting molecular ions which have been chemically charged in a predetermined manner to time-of-flight or Fourier transform mass spectroscopy.
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